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The painting's appearance in the late 1970s was badly deteriorated. From 1978 to 1999 Pinin Brambilla Barcilon guided a major restoration project which undertook to permanently stabilize the painting, and reverse the damage caused by dirt, pollution, and the misguided 18th and 19th century restoration attempts. Since it had proved impractical to move the painting to a more controlled environment, the refectory was instead converted to a sealed, climate controlled environment, which meant bricking up the windows. Then, detailed study was undertaken to determine the painting's original form, using scientific tests (especially infrared reflectoscopy and microscopic core-samples), and original cartoons preserved in the Royal Library at Windsor Castle. Some areas were deemed unrestorable. These were re-painted with watercolour in subdued colours intended to indicate they were not original work, whilst not being too distracting.
This restoration took 21 years and on May 28, 1999 the painting was put back on display, although intending visitors are required to book ahead and can only stay for 15 minutes. When it was unveiled, considerable controversy was aroused by the dramatic changes in colours, tones, and even some facial shapes. James Beck, professor of art history at Columbia University and founder of ArtWatch International, had been a particularly strong critic.
resources: wikipedia.org
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